Although quite widely stated, as I know from my own searches for information on the subject, the above answer is quite wrong. The July 2010 27-inch models have three inboard SATA connectors (though not the 21.5-inch models). The third port is difficult to reach, but by no means impossible if you're handy with suction cups and a torx driver. If you don't have the 2010 27-inch iMac built to order with an SSD from Apple, you'll be missing some necessary cabling and a mounting bracket, but there are ways around that. I'd suggest checking out the following.
I upgraded my own 2010 27-inch with a third-party 2.5-inch SSD using the second site as a guide for parts and procedures.
The 2009 27-inch iMacs do NOT have the third SATA port with which the 2010 models are appointed.
Hope that helps!
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